|
KMID : 0880220150530040250
|
|
Journal of Microbiology
2015 Volume.53 No. 4 p.250 ~ p.255
|
|
|
Effect of promoter-upstream sequence on σ38-dependent stationary phase gene transcription
|
|
Lim Hyung-Ju
Kim Kwang-Soo
Shin Min-Sang
Jeong Jae-Ho
Ryu Phil-Youl
Choy Hyon-E.
|
|
|
Abstract
|
|
|
|
σ38 in Escherichia coli is required for expression of a subset of stationary phase genes. However, the promoter elements for σ38-dependent genes are virtually indistinguishable from that for σ70-dependent house-keeping genes. hdeABp is a σ38-dependent promoter and LEE5p is a σ70-dependent promoter, but both are repressed by H-NS, a bacterial histone-like protein, which acts at promoter upstream sequence. We swapped the promoter upstream sequences of the two promoters and found that the σ dependency was switched. This was further verified using lacUV5 core promoter. The results suggested that the determinant for σ38-dependent promoter lies in the promoter upstream sequence.
|
|
|
KEYWORD
|
|
|
E. coli, promoter specificity, RNA polymerase, rpoS, σ38
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|